Study of peculiarities of enantiomeric separation of beta-blockers by using of polysaccharide-based chiral columns

Author: marina karchkhadze
Co-authors: Lali Chankvetadze, Antonina Mskhiladze, Izabella Giuashvili, Nino Takaishvili, Natalia Okujava, Bezhan Chankvetadze
Keywords: liquid chromatography, polysaccharide chiral columns, separation of enantiomers, elution order, chiral beta-blockers.
Annotation:

Beta – blockers represent chiral compounds with basic nature, which are widely used in medical practice at cardiac insufficiency. The therapeutic effect of beta-blockers are determined by action of their one enantiomer. Therefore, enantiomeric purity is very important. The aim of our work was investigation of separation process of beta-blockers enantiomers on polysaccharide-based chiral stationary columns by using of various mobile phases. Separation of the enantiomers of 28 beta – blockers was studied on polysaccharide chiral phases by hexane - isopropanol eluents with different ratio in the presence of additive of diethylamine. The influence of the nature of the stationary phase, the composition of mobile phase and temperature on the separation of enantiomers and elution order was established. The change of elution order takes place in some cases, viz., the elution order of sotalol is reversed on Cellulose-1 and Amylose-2 columns. The analogous picture is obtained for penbutalol and timolol on Cellulose-1 and Cellulose-2 chiral columns, which differ from each other by the nature and location of substituents. The change of composition of the mobile phase results in the reversion of the elution order. The elution order of bupranolol is changed by decreasing of the volume fraction of isopropanol in the mobile phase.